The experimental programme is divided into three phases, the Pilot Study, Cross-Sectional Study, and the Longitudinal Study. The research involves physico-chemical characterisation of pit latrines, adjacent soil assessment and biochemical for key makers of decomposition. As part of a wider collaboration we will be preparing samples for specific techniques only available in a few locations, such as DNA sequencing (Sanger Institute, Cambridge) and biodegradability (Wageningen University). This program covers a period of July 2012.
During the pilot study (2 months) we will test out our sampling equipment, establish our laboratory protocols, and prepare the larger cross-sectional study. We will also develop good working relationships between the LSHTM and IHI team members and undertake any training necessary of staff working on the project.
During the cross-sectional study (10 months) will use the methods developed during the pilot but will be made on a much larger number of pits - up to 50 in total. This will allow us to look at a range of pits differing in performance and user behaviour.
During the longitudinal study (12 months), pits selected from the cross-sectional study will be followed for up to 12 months using techniques judged to be most relevant and informative from earlier results. It is expected that the frequency of sampling will be monthly.